Rapid fluorogenic assay for differentiation of the Candida parapsilosis group from other Candida spp.
نویسندگان
چکیده
A 30-min fluorogenic test was developed for differentiation of members of the Candida parapsilosis group from other Candida species commonly encountered in clinical material. The fluorogenic substrate, 4-methylumbelliferyl-beta-D-glucoside, was utilized to assay beta-glucosidase activity. A total of 50 C. parapsilosis isolates and 135 isolates of four other Candida species were tested. Assay sensitivity and specificity were 100 and 99.3%, respectively. The procedure was adapted for use with a spectrofluorometer.
منابع مشابه
Enzymatic differentiation of Candida parapsilosis from other Candida spp. in a membrane filtration test.
A previously reported enzyme assay on a membrane filter using 4-methylumbelliferyl (4-MU)-N-acetyl-beta-D-galactosaminide, -phosphate and -pyrophosphate as substrates for the differentiation of four Candida spp. has been extended to Candida parapsilosis. The substrate 4-MU-beta-D-glucoside was hydrolyzed by 28 test strains of this species but to a variable extent by seven other yeasts also. For...
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 27 1 شماره
صفحات -
تاریخ انتشار 1989